EBV VCA Panel IgM


EBV VCA Panel IgM

(segue)Human B lymphocytes, EBV target cells, present on their membrane a receptor, Cr2 (CD21), to which the glycoproteins gp325 and gp42 of the virus bind. This receptor is also the point on which the complement C3d fragment is bound. So EBV uses a physiological receptor, imitating a natural bond and succeeding in circumventing the defenses of the host. A similar situation is that between the HIV virus and the CD4 molecule of T lymphocytes. The infection of other types of cells is much less efficient and occurs through poorly known pathways. After it has been fixed to its receptor, EBV penetrates into lymphocyte B and its DNA persists in the cell in two forms: an episomal form, in which viral DNA remains circular, detached from genomic material human, and an integrated form where DNA is incorporated into the host genome, without however a specific integration site. The Epstein-Barr virus is potentially oncogenic and has been related to many diseases; is also a major problem in AIDS patients, where it is associated with widespread polyclonal lymphomas, interstitial lymphocytic pneumonia, and oral cavity leukoplakia. Burkitt’s lymphomas Positive EBVs and other viral-related lymphoproliferative diseases (generally referred to as the PTLD acronym) are increasingly observed in transplant recipients receiving immunosuppressive therapy. In a healthy individual, the latent state of EBV infection is monitored by humoral immunity, cytotoxic T lymphocytes and interferon system.

Sensitivity

Specificity
Positive control
 

Broad antigen from bovine erythrocytes, low level 1

 

99.6%

 

100%

 

Broad antigen from bovine erythrocytes, level 2 high

 

98.7%

 

100%

 

EBV-VCA (gp125, purified)

 

99.3%

 

100%

 

CMV (Lysate of strain glycine AD-169)

 

100%

 

100%

 

negative control

 

Total reaction time: 100/140 minutes at 44-47 ° C.

First incubation: 60/90 minutes

Second incubation: 5 minutes

Third incubation: 30/40 minutes

First incubation: 5 minutes

Ref:                 800-6025                                25 tests